Laser capture microdissection
Laser microdissection enables te visual identification of cells using microscopy and the subsequent isolation of these cells or of cell clustures for downstream analysis.
LCM systems at Ghent University
The Ghent University LCM core has two systems: the Arcturus Pixcell II system and the Zeiss PALM Microbeam IV system
Pixcell II versus PALM
Laser capture microdissection
Laser catapulting
Laser capture microdissection enables isolation of cells from tisseu sections using a cap with a thermoplastic polymer film. The infrared laser is pointed on the cells of interests and melts the polymer onto these cells. by lifting the cap, the cells of interest that adhere to the melted polymer are isolated and can be used for further analysis.
Laser catapulting enables the isolation of cells by using a UV laser to cut and catapult cells of interest into an overlaying cap. This system has the advantage that no contamination of surrounding tissue is possible.
